Ÿ ƒRƒhƒ“‚Ęˆā“`ˆĆ†•\ Ÿ
Ø ƒgƒsƒbƒNƒXiRNA‚Ģ˜b‘č‚Č‚Ē‚šŠÜ‚Žj b ‹Œ”Łiƒuƒ‰ƒEƒU‚ĶIE‚Łj b Jmol ƒRƒ“ƒeƒ“ƒcƒŠƒXƒg a Chime”Łi•ŖŽq•\Ž¦’†Sj ©

2006”N‚Ģƒm[ƒxƒ‹¶—ŠwˆćŠwÜ‚ĶRNAŠ±Ā‚ĢŒ¤‹†‚É!!
The Nobel Prize in Physiology or Medicine 2006 (Nobel Foundation) b Press Release
¦”Œć‚Ģī•ń’Ē‰Į‚Ķ2006”Nƒm[ƒxƒ‹¶—ŠwˆćŠwÜFRNAŠ±Ā‚ōs‚¢‚Ü‚·!!


ƒRƒhƒ“‰š–¾‚Ģ‚«‚Į‚©‚Æ‚Ę‚Č‚Į‚½ŽĄŒ±‚ÉŠÖ˜A‚µ‚Ä

mRNAiUUUUUUUUUUUUjGƒj[ƒŒƒ“ƒo[ƒO‚ĢŽĄŒ±
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
DNA/RNA‘I‘š@“Æbackbone‘I‘š
‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
CPKF
@
”wŒiE•@ŠD@”’@®

mRNAiCACACACACACAjGƒRƒ‰[ƒi‚ĢŽĄŒ±
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
DNA/RNA‘I‘š@“Æbackbone‘I‘š
‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
CPKF
@
”wŒiE•@ŠD@”’@®


mRNAiUUUUUUUUUUUUj‚ō‡¬‚³‚ź‚éƒ|ƒŠƒyƒvƒ`ƒhi•”•Ŗj
CPKF@Ž_«E’†«E‰–Šī«@I/O’l‡
”wŒiE•@ŠD@”’@®
‹óŠŌ[“U@‹…–_@‹…60“
¦ŽQlFNature ”T‚ĢƒnƒCƒ‰ƒCƒgi2010/06j

mRNAiCACACACACACAj‚ō‡¬‚³‚ź‚éƒ|ƒŠƒyƒvƒ`ƒhi•”•Ŗj
CPKF@Ž_«E’†«E‰–Šī«@I/O’l‡
”wŒiE•@ŠD@”’@®
‹óŠŌ[“U@‹…–_@‹…60“
¦ŽQlFNature ”T‚ĢƒnƒCƒ‰ƒCƒgi2010/06j



PDB_1g59‚ĢChain AEBitRNAGlu‚ĘGluRS‚Ģ•”‡‘Ģj
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
@
@
”wŒiE•@ŠD@”’@®

PDB_1asy‚ĢChain AERitRNAAsp‚ĘAspRS‚Ģ•”‡‘Ģj
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
@
@
”wŒiE•@ŠD@”’@®

PDB_1ml5‚ĢChain BECiP-site tRNAPhe‚ĘA- and P-site mRNA codonsj
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
@
@
”wŒiE•@ŠD@”’@®

PDB_1gix‚ĢChain BECE1iA-site‚Ø‚ę‚ŃP-site tRNAPhe‚ĘA- and P-site mRNA codonsj
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
@
@
”wŒiE•@ŠD@”’@®

PDB_1wz2‚ĢChain AEC
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
@
@
”wŒiE•@ŠD@”’@®

PDB_1wz2‚ĢChain AEC
ƒoƒbƒNƒ{[ƒ“@“ńŽŸ\‘¢@DNA/RNAiATGCUCbackbonej
‘S‘I‘š@ƒ^ƒ“ƒpƒNŽæ‘I‘š@ƒŠƒKƒ“ƒh‘I‘š
DNA/RNA‘I‘š@“Æbackbone‘I‘š

‹óŠŌ[“U@‹…–_@‹…60“@ƒXƒeƒBƒbƒN@j‹ą
ƒAƒ~ƒmF@ChainF@CPKF@a@JmolF@RasmolF

Ž_«E’†«E‰–Šī«‹ę•Ź@‹É«E”ń‹É«‹ę•Ź
‘a…«ƒCƒ“ƒfƒbƒNƒX‡@I/O’l‡i“Į«Šī Rj@“™“d“_‡
ƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«iƒæƒwƒŠƒbƒNƒXEƒĄƒXƒgƒ‰ƒ“ƒhj
…‘fŒ‹‡•\Ž¦
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}1@ƒZƒ“ƒgƒ‰ƒ‹ƒhƒOƒ}‚ĘRNAƒ[ƒ‹ƒh
¦ˆČ‰ŗ‚ÉŒfŚ‚Ģ}‚š‰ü•ĻG–öģOŽuCu¶–½‚Ķ‚¢‚©‚É‘n‚ē‚ź‚½‚©vCTBSƒuƒŠƒ^ƒjƒJ(1991)



}2@tRNA‚šŠÜ‚ŽPDBƒf[ƒ^—įi1g59‚ę‚čCPDB•”•Ŗƒf[ƒ^ƒŠƒXƒg‚É‚ąŽū˜^jGŠg‘å
Glutamyl-tRNA Synthetase Complexed With tRNA(Glu).
m¶ntRNA‚ĢLŽšŒ^ŽOŽŸ\‘¢‚ĢœŠiCm’†n1g59‚ĢChain BitRNAGlujCm‰En1g59‚ĢChain AEBitRNAGlu‚ĘGluRS‚Ģ•”‡‘Ģj
¦’†E‰E‚Ģ}‚Å‚ĶCRNA‚Ķ‹…–_i‰–Šī‚ĢF‚Ķ‰ŗ•\‚Ę“Æ‚¶jCƒ^ƒ“ƒpƒNŽæ‚ĶƒXƒeƒBƒbƒNiF‚Ķ‰ŗ•\aminoŒ`Ž®j•\Ž¦
’†‘ŗ‹`ˆź •ŅCuRNA‚Ŗ‚ķ‚©‚évCp.90C—r“yŽŠ(2003)


mRNA‚Ģˆā“`ˆĆ†•\
¦¶•ØŽķ‚É‚ę‚Į‚Ä‚ĶCˆČ‰ŗ‚Ģ••ÕˆĆ†‚ɏ]‚ķ‚Č‚¢ƒRƒhƒ“‚šŽ‚Āź‡‚ą‚ ‚éB
  ‘ę‚Q•¶Žš  
U C A G
‘ę
‚P
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U UUU Phe^F UCU Ser^S UAU Tyr^Y UGU Cys^C U ‘ę
‚R
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UUC Phe^F UCC Ser^S UAC Tyr^Y UGC Cys^C C
UUA Leu^L UCA Ser^S UAA I—¹ UGA I—¹ A
UUG Leu^L UCG Ser^S UAG I—¹ UGG Trp^W G
C CUU Leu^L CCU Pro^P CAU His^H CGU Arg^R U
CUC Leu^L CCC Pro^P CAC His^H CGC Arg^R C
CUA Leu^L CCA Pro^P CAA Gln^Q CGA Arg^R A
CUG Leu^L CCG Pro^P CAG Gln^Q CGG Arg^R G
A AUU Ile^I ACU Thr^T AAU Asn^N AGU Ser^S U
AUC Ile^I ACC Thr^T AAC Asn^N AGC Ser^S C
AUA Ile^I ACA Thr^T AAA Lys^K AGA Arg^R A
AUG Met^MiŠJŽnj ACG Thr^T AAG Lys^K AGG Arg^R G
G GUU Val^V GCU Ala^A GAU Asp^D GGU Gly^G U
GUC Val^V GCC Ala^A GAC Asp^D GGC Gly^G C
GUA Val^V GCA Ala^A GAA Glu^E GGA Gly^G A
GUG Val^V GCG Ala^A GAG Glu^E GGG Gly^G G


DNA‚Ģˆā“`ˆĆ†•\(1)
¦•¶ŽšF‚ĶJmol‚ĢaminoFić•\‚Ę“Æ‚¶jB
  ‘ę‚Q•¶Žš  
T C A G
‘ę
‚P
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T TTT Phe^F TCT Ser^S TAT Tyr^Y TGT Cys^C T ‘ę
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TTC Phe^F TCC Ser^S TAC Tyr^Y TGC Cys^C C
TTA Leu^L TCA Ser^S TAA I—¹ TGA I—¹ A
TTG Leu^L TCG Ser^S TAG I—¹ TGG Trp^W G
C CTT Leu^L CCT Pro^P CAT His^H CGT Arg^R T
CTC Leu^L CCC Pro^P CAC His^H CGC Arg^R C
CTA Leu^L CCA Pro^P CAA Gln^Q CGA Arg^R A
CTG Leu^L CCG Pro^P CAG Gln^Q CGG Arg^R G
A ATT Ile^I ACT Thr^T AAT Asn^N AGT Ser^S T
ATC Ile^I ACC Thr^T AAC Asn^N AGC Ser^S C
ATA Ile^I ACA Thr^T AAA Lys^K AGA Arg^R A
ATG Met^MiŠJŽnj ACG Thr^T AAG Lys^K AGG Arg^R G
G GTT Val^V GCT Ala^A GAT Asp^D GGT Gly^G T
GTC Val^V GCC Ala^A GAC Asp^D GGC Gly^G C
GTA Val^V GCA Ala^A GAA Glu^E GGA Gly^G A
GTG Val^V GCG Ala^A GAG Glu^E GGG Gly^G G
œƒAƒ~ƒmF•\Ž¦‚Ģ–}—į
ASP@GLU@CYS@MET@LYS@ARG@SER@THR@PHE@TYR@ASN@GLN@GLY@LEU@VAL@ILE@ALA@TRP@HIS@PRO


DNA‚Ģˆā“`ˆĆ†•\(2)
¦ƒZƒ‹‚ĢF‚Ķ—L‹@ŠT”O}I/O’l‡i“Į«Šī RjC•¶ŽšF‚Ķ‘a…«ƒCƒ“ƒfƒbƒNƒX‡B
•Źƒy[ƒWˆź——•\ŽQĘB
  ‘ę‚Q•¶Žš  
T C A G
‘ę
‚P
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T TTT Phe^F TCT Ser^S TAT Tyr^Y TGT Cys^C T ‘ę
‚R
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TTC Phe^F TCC Ser^S TAC Tyr^Y TGC Cys^C C
TTA Leu^L TCA Ser^S TAA I—¹ TGA I—¹ A
TTG Leu^L TCG Ser^S TAG I—¹ TGG Trp^W G
C CTT Leu^L CCT Pro^P CAT His^H CGT Arg^R T
CTC Leu^L CCC Pro^P CAC His^H CGC Arg^R C
CTA Leu^L CCA Pro^P CAA Gln^Q CGA Arg^R A
CTG Leu^L CCG Pro^P CAG Gln^Q CGG Arg^R G
A ATT Ile^I ACT Thr^T AAT Asn^N AGT Ser^S T
ATC Ile^I ACC Thr^T AAC Asn^N AGC Ser^S C
ATA Ile^I ACA Thr^T AAA Lys^K AGA Arg^R A
ATG Met^MiŠJŽnj ACG Thr^T AAG Lys^K AGG Arg^R G
G GTT Val^V GCT Ala^A GAT Asp^D GGT Gly^G T
GTC Val^V GCC Ala^A GAC Asp^D GGC Gly^G C
GTA Val^V GCA Ala^A GAA Glu^E GGA Gly^G A
GTG Val^V GCG Ala^A GAG Glu^E GGG Gly^G G
œ‘a…«ƒCƒ“ƒfƒbƒNƒX‡
ARG@LYS@ASN@ASP@GLN@GLU@HIS@PRO@TYR@TRP@SER@THR@GLY@ALA@MET@CYS@PHE@LEU@VAL@ILE
œ—L‹@ŠT”O}I/O’l‡i“Į«Šī Rj
ASN@SER@ASP@GLN@GLU@THR@ARG@HIS@GLY@LYS@TYR@TRP@CYS@MET@PRO@PHE@ALA@VAL@LEU@ILE


ƒAƒ~ƒmŽ_ŽcŠī‚Ģ‘a…«ƒCƒ“ƒfƒbƒNƒX‚ĘI'/O'’l‚Ģ”äŠriGly‚šœ‚­j


¦ŽQliŒg‘Ń”ÅƒRƒhƒ“•\j Ø mRNACDNA


œ˜b‘č‚PFtRNA‹[‘Ōƒ^ƒ“ƒpƒNŽæ


}‚R@tRNA‹[‘Ōƒ^ƒ“ƒpƒNŽæ‚Ģ—įi‰ŗ‹L•¶Œ£p.21}2‚šŽQl‚ɍģ¬GPDB•”•Ŗƒf[ƒ^ƒŠƒXƒg‚É‚ąƒf[ƒ^Žū˜^j
mćn¶‚©‚ēCRRFGi1eh1_AjCeRF1i1dt9_Aj
m’†n¶‚©‚ēCEF-Gi1dar_AjCtRNAi‚±‚±‚Å‚Ķ1ml5_BCjCEF-Tu tRNA •”‡‘Ģi1ttt_ADj
m‰ŗnRF2i1gqe_AGŒ“•ń‚Ę•ŖŽq‚ĢŒü‚«‚ŖˆŁ‚Č‚éj


œ˜b‘č‚QF[GADV]-ƒ^ƒ“ƒpƒNŽæƒ[ƒ‹ƒh‰¼ą


}4 [GADV]-ƒ^ƒ“ƒpƒNŽæƒ[ƒ‹ƒh‰¼ą


GNCŒ“‰ˆā“`ˆĆ†
  ‘ę‚Q•¶Žš  
U C A G
‘ę
‚P
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G GUC Val^V GCC Ala^A GAC Asp^D GGC Gly^G C ‘ę
‚R
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¦’·Ą ‹BEˆä“c –ĪCu’n‹…ŠO¶–½@‚ķ‚ź‚ķ‚ź‚ĶŒĒ“Ę‚©vCŠā”gV‘(2014)‚Ģp.39‚Å‚ąŠ‰īB


ƒAƒ~ƒmŽ_‚ĢƒRƒ“ƒzƒ[ƒVƒ‡ƒ“‘I‘š«ip.41}9ŠÖ˜Aj
¦
uƒ^ƒ“ƒpƒNŽæ‚Ģ\‘¢‚Ę‹@”\ vCƒƒfƒBƒJƒ‹EƒTƒCƒGƒ“ƒXEƒCƒ“ƒ^[ƒiƒVƒ‡ƒiƒ‹(2005)@c}1-20‚Ģ•\‚©‚ē‹tƒpƒ^[ƒ“‚Ģ”’l‚šœ‚¢‚½‚ą‚Ģ
¦‘ę1Ķw”z—ń‚©‚ē\‘¢‚ցxFŒ“•¶iFrom Sequence to Structurej[PDF]
¦Œ³ƒf[ƒ^F Williams, R.W. et al. : Biochim. Biophys. Acta , 916, 200-204 (1987).
¦ŽQlF‚P•¶ŽšƒAƒ~ƒmŽ_”z—ń‚©‚ēŠeŽķHTMLƒf[ƒ^‚šģ¬‚·‚éGŠŪƒ}ƒNƒ
  ƒæ-ƒwƒŠƒbƒNƒX ƒĄ-ƒXƒgƒ‰ƒ“ƒh
Glu 1.59 0.52
Ala 1.41 0.72
Leu 1.34 1.22
Met 1.30 1.14
Gln 1.27 0.98
Lys 1.23 0.69
Arg 1.21 0.84
His 1.05 0.80
Val 0.90 1.87
Ile 1.09 1.67
Tyr 0.74 1.45
Cys 0.66 1.40
Trp 1.02 1.35
Phe 1.16 1.33
Thr 0.76 1.17
Gly 0.43 0.58
Asn 0.76 0.48
Pro 0.34 0.31
Ser 0.57 0.96
Asp 0.99 0.39


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